Activated Protein C Assay / Antithrombin (ATIII) / Anticardiolipin Antibodies (IgG and IgM) / Beta-2 Glycoprotein Antibodies, IgG & IgM / Lupus Anticoagulant / Protein C Activity / Protein S Activity / Prothrombin Gene Mutation.

Specimen Collection Requirements


Total of six tubes collected: Four 4.5 mL light blue top tubes (sodium citrate) AND one 4 mL lavender top tube (EDTA) AND one 5 mL red top tube or 7.5 mL serum separator tube (SST). Collect by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill blue tubes to maximum draw, at least 90% full. Mix by gentle inversion.


Due to short stability, specimens must either be sent STAT to arrive at the laboratory within 4 hours, or sent frozen. For STAT, refrigerate whole blood in unopened tubes and call for STAT pick-up. For standard delivery, centrifuge blue tubes to produce platelet-poor plasma (at least 1700 X g for 15 minutes to produce PLT CT less than 10,000 per cumm) and using a plastic pipette, transfer the top 2/3 of the plasma into 3-4 plastic vials. Label each tube of citrated plasma with patient name, I.D. number, test, and “citrated plasma.” Freeze immediately. Centrifuge the red top tube and transfer serum into two tubes, each labeled with patient name, test, and “serum.” The EDTA tube must be submitted as unopened refrigerated whole blood. Send to the laboraotry within 7 days. If multiple tests are ordered, freeze a separate aliquot for this test.

Preferred Volume

Four 4.5 mL light blue top tubes (or 4 mL plasma) AND one 4 mL lavender top tube (whole blood), AND one 5 mL red top tube (or 1 mL serum).

Minimum Volume

Two 2.7 mL light blue top tubes (or 2 mL plasma) AND one 4 mL lavender top tube (whole blood) AND one 5 mL red top (or 1 mL serum).


Original tubes refrigerated if sent STAT; citrated plasma frozen on dry ice; whole blood and serum refrigerated.)

Rejection Criteria

Clotted samples, samples collected in containers other than 3.2% Buffered Sodium Citrate, tubes filled less than 90%, samples from patients with a Hematocrit greater than 55%, hemolyzed samples due to ex-vivo causes (i.e., difficult draw, poor sample draw), whole blood samples that were frozen before processing


Original tubes: 4 hours refrigerated; citrated plasma: 7 days frozen; whole blood: 7 days refrigerated.

Testing Information


Mon, Wed, Fr


Chromogenic, Clotting, Molecular

Referral Lab

Legacy Laboratory Services

Reference Values

Reference Values

See individual test

CPT Codes

83891-3G / 83896-3G x2 / 83898-3G / 83903-3G / 83912-3G / 85300 / 85303 / 85306 / 85307 / 85613 / 85730 / 86146 x2 / 86147 x2

Last Updated: Thursday, January 5, 2017 03:42:31 AM