Hypercoag Panel - Thrombophilia Panel - Thrombotic Risk Panel
Activated Protein C Resistance / Cardiolipin Antibodies, IgG and IgM / Prothrombin Gene Mutation
Specimen Collection Requirements
Total of six tubes collected: Four 4.5 mL light blue top tubes (sodium citrate) AND one 5 mL lavender top tube (EDTA) AND one 5 mL red top (clot) OR one 7.5 mL serum separator tube (SST). Collect by careful venipuncture to avoid tissue fluid contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill blue tubes to maximum draw, at least 90% full. Mix by gentle inversion.
Due to short stability, specimens must either be sent STAT to arrive at the laboratory within 4 hours, or sent frozen. For STAT, refrigerate whole blood in unopened tubes and call for STAT pick-up. For standard delivery, centrifuge blue tubes to produce platelet-poor plasma (at least 1700 X g for 15 minutes to produce PLAT CT less than 10,000 per cumm) and using a plastic pipette transfer the top 2/3 of the plasma into 3-4 plastic vials. Freeze immediately. Label citrated plasma with patient name, I.D. number, test, and "plasma." Serum from the red top tube can be centrifuged and transferred to a single tube, labelled with patient name, I.D. number, test, and "serum." The EDTA tube must be submitted as refrigerated whole blood.
Four 4.5 mL light blue top tubes (or 4 mL plasma) AND one 3 mL red top tube (or 1 mL serum) AND one 5 mL lavender top tube (whole blood).
Two 2.7 mL light blue top tubes (or 2 mL plasma) AND one 2 mL lavender top tube (whole blood).
Original tubes refrigerated if sent STAT; citrated plasma frozen on dry ice; whole blood and serum refrigerated. )
Clotted samples, samples collected in containers other than 3.2% Buffered Sodium Citrate, tubes filled less than 90%, samples from patients with a Hematocrit greater than 55%, hemolyzed samples due to ex-vivo causes (i.e., difficult draw, poor sample draw), whole blood samples that were frozen before processing
WHOLE BLOOD Ambient: 4 hours; PLASMA Ambient: 4 hours; Frozen: -20°C 2 weeks, -70°C 6 months
Legacy Laboratory Services
Thursday, January 5, 2017 03:42:31 AM